Three-dimensional (3D) printing is a simple, attractive route for prototyping and fabricating bioanalytical devices that require many modifications along the road to optimization. This prototype device holds good promise for further development as a point-of-care cancer diagnostics tool. Good accuracy vs ELISA was validated by analyzing human serum samples. Detection limits of 0.5 pg mL −1 were achieved for these proteins in diluted serum with log dynamic ranges of four orders of magnitude. The device was used for multiplexed detection of prostate cancer biomarker proteins prostate specific antigen (PSA) and platelet factor 4 (PF-4). Total assay time was ~30 min in a complete automated assay employing a programmable syringe pump so that the protocol required minimal operator intervention.
Sandwich type assays were built onto the glass arrays using a multi-labeled detection antibody-polyHRP (HRP = horseradish peroxidase). The unibody device features three reagent reservoirs, an efficient 3D network for passive mixing, and an optically transparent detection chamber housing a glass capture antibody array for measuring chemiluminescence output with a CCD camera. A low cost three-dimensional (3D) printed clear plastic microfluidic device was fabricated for fast, low cost automated protein detection.
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